Effect of Non Volatile Compounds of Trichoderma Spp. against Fusarium Graminearum, Rhizoctonia Solani and Pythium Ultimum

Biological control represents an important approach of agricultural biotechnology for controlling many fungal plant pathogens. Trichoderma spp. are the most promising and effective bioagents against many plant pathogenic fungi. In present paper, two strains of Trichoderma aspellerum isolated from soil were screened for their efficacy against some common soil borne plant pathogens by dual culture technique. Trichoderma strains were grown in potato dextrose broth and collected metabolites were amended to PDA medium to obtain 5, 10, 25 and 50% concentration in Petri plates. The solidified agar plates were inoculated with pathogen and incubated at 25 ± 2oC for 7 days. The colony diameter was measured and percentage inhibition of radial growth was calculated. Both antagonists’ strains produced non-volatile metabolites and inhibit the mycelial growth of Fusarium graminearum, Rhizoctonia solani and Pythium umtimum.